A Cross-Sectional Study of the Frequency of Rhesus Factor in the Population of Islamabad

  • Madeeha Subhan Waleed Ayub Medical College, Pakistan.
  • Sanniyah Subhan Oakton Community College, Chicago, IL, USA.
  • Waleed Sadiq Staten Island University Hospital, New York, NY, USA.
Keywords: Rh Factor, Rhesus Factor, ABO.


Background: Rh factor is called the "Rhesus factor" because it was first discovered in the blood of Rhesus monkeys. Rh factor is an antigen, a substance that stimulates the production of antibodies to fight foreign agents such as viruses, bacteria, and transplanted organs. Besides its role in blood transfusion, the D antigen is used to determine the risk of hemolytic disease of the newborn (or erythroblastosis fetalis) for Rh disease management.

Method: After obtaining informed consent from the couple presented to the National Institute of Health Islamabad, blood grouping was carried out using standardized agglutination techniques. Blood samples were drawn aseptically and transferred to ethylenediaminetetraacetic acid-containing tubes. Slide agglutination testing was performed to determine Rh factor D (RhD).

Results: A total of 111 of 116 couples had the same Rh factors; 105 couples were Rh-positive, and six couples were Rh-negative.